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KMID : 0368420070500010029
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Journal of Plant Biology
2007 Volume.50 No. 1 p.29 ~ p.37
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Aptamers improve the expression of a human granulocyte-macrophage colony stimulating factor in transgenicArabidopsis thaliana seeds
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Wang Biao
Ma Ming
Wu Tian Long
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Abstract
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Domain IV nucleotides of human 7SL RNA comprise an approximately 50-nucleotide region with a highly conserved secondary structure consisting of two internal loops. We inserted these into a modified 3’-untranslated region of the human granulocyte-macrophage colony stimulating factor (hGM-CSF) gene. The expression vector, under the control of a tissue-specific promoter derived from the soybean α’ subunit of β-conglycinin, was introduced intoArabidopsis thaliana byAgrohacterium-mediated transformation. Expression of the recombinant hGM-CSF significantly improved, accounting for as much as 0.049% of the total soluble protein in theArabidopsis siliques. Western blots showed that the molecular weight of this recombinant hGM-CSF was approximately 21 kD. In addition, TF-1 cell proliferation data demonstrated that the recombinant hGM-CSF was biologically active. Our results provide evidence that aptamers can strongly enhance gene expression.
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KEYWORD
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aptamers, human GM-CSF, TF-1 cell
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